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Hardware

We recommend using a system with at least an 8-core Intel or AMD x86_64 processor.

Memory requirements depend heavily on the input data and the level of parallelism. However, we suggest having at least one node with 512 GB of RAM available.

Disk capacity at runtime should be several times larger than the total size of the FASTQ library files to be processed.

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ARM processors (e.g. Apple M series, Amazon Graviton, Ampere) are currently not supported.

Operating System

nf-core/pixelator can be run on any POSIX-compatible system, such as Linux or macOS.
On Windows, the pipeline can be executed using the WSL2 integration layer.

Software

Nextflow requires Bash version 3.2 or later, and Java 17 (up to Java 24) to be installed.